Genotoxic Effects of Cobalt Chloride on Mitotic Index of Allium cepa L.
Keywords:
Genotoxic, Mitotic Index, C-mitosis, Allium, Budding nucleus, Heavy metalAbstract
Root meristem cells are highly sensitive to genetic damage by chemicals; hence, present work is undertaken to assess the effect of different concentrations of Cobalt chloride on mitotic index of Allium cepa L. Experiments were conducted by using onion bulbs. These bulbs are planted in paper cups containing sand and soil mixture (2:1 ratio) and plants were treated with different molarity (M) of Cobalt chloride. The roots were macerated to analyze the mitotic index and chromosomal abnormalities. The mitotic index was 11.55%, 11.33% and 10.988% for the treatment with 0.5, 1.0, and 1.5M CoCl2 respectively and it was least (9.1%) with 2.5M of CoCl2. Maximum interphase cells (409) were observed with 2.5M, compared with the control and other treatments (0.5M: 398 cells, 1.0M: 399 cells and 1.5M 401 cells). The plants treated with 1.5M and 2.0M CoCl2 have the highest number of metaphase cells (16) and less number of cells with anaphase observed in plant roots treated 2.5M CoCl2 (2 cells) followed by 0.5M and 2.0M CoCl2. The total chromosomal abnormalities in the root tips treated with 2.5M CoCl2 are 21(51.2%) was observed. Micronucleus of budding nucleus have an approximate half percentage 9 (21.9) whereas C-mitosis and chromosome stainless have the same proportion 3(7.3). It was noted that, the unequal sized 5 nuclei (12.1%), the anaphase bridge has the lowest percentage 1(2.04%) on the other hand 2.5M CoCl2 conc., has only 1(1.91) of C-mitosis and chromosome stainless. The study highlights that, the higher concentration of heavy metals have negative effect on the mitotic index of root meristematic cells.
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Abubacker, M. N., & Sathya, C. (2017). Genotoxic effect of heavy metals Cr, Cu, Pb and Zn using Allium cepa L. Biosciences Biotechnology Research Asia, 14(3), 1181–1186.
Awe, E. T., & Akpan, U. U. (2017). Cytological study of Allium cepa and Allium sativum. Actosa Technica, 9(1), 113–120.
El-Shahaby, A. O., Abdel Migid, H. M., Soliman, M. I., & Mashaly, I. A. (2003). Genotoxicity screening of industrial wastewater using the Allium cepa chromosome aberration assay. Pakistan Journal of Biological Sciences, 6, 23–28.
Fenech, M., Kirsch-Volders, M., Natarajan, A. T., Surrallés, J., Crott, J. W., Parry, J., ... & Norppa, H. (2011). Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis, 26(1), 125–132.
Fernandes, T. C. C., Mazzeo, D. E. C., & Marin-Morales, M. A. (2007). Mechanism of micronuclei formation in polyploidized cells of Allium cepa exposed to trifluralin herbicide. Pesticide Biochemistry and Physiology, 88, 252–259.
Firbas, P., & Amon, T. (2014). Chromosome damage studies in the onion plant Allium cepa L. International Journal of Cytology, Cytosystematics and Cytogenetics, 67(1), 25–35.
Hajmoradi, F., & Kakei, M. (2021). Genotoxic effects of heavy metals on mitotic chromosomes of Trigonella foenum-graecum L. Journal of Genetic Resources, 7(2), 265–271.
Lauchli, L., & Epstein, E. (1990). Plant response to saline conditions. In Agricultural salinity assessment and management (Vol. 15, pp. 113–137).
Lei, M., Tie, B.-Q., Song, Z.-G., Liao, B.-H., Lepo, J. E., & Huang, Y.-Z. (2015). Heavy metal pollution and potential health risk assessment of white rice around mine areas in Hunan Province, China. Food Security, 7, 45–54.
Leme, D. M., & Marin-Morales, M. A. (2009). Allium cepa test in environmental monitoring: A review on its application. Mutation Research, 682, 71–81.
Lopez-Millan, A., Sagardoy, R., Solanas, M., Abadia, A., & Abadia, J. (2009). Cadmium toxicity in tomato (Lycopersicon esculentum) plants grown in hydroponics. Environmental and Experimental Botany, 65, 376–385.
Munns, R. (2002). Comparative physiology of salt and water stress. Plant, Cell & Environment, 25, 239–250.
Pohren, R., Thatiana, C., & Vargas, V. M. F. (2013). Investigation of sensitivity of the Allium cepa test as an alert system to evaluate the genotoxic potential of soil contaminated by heavy metals. Water, Air, & Soil Pollution, 224, 1460–1470.
Zhang, X., Zhong, T., Liu, L., & Ouyang, X. (2015). Impact of soil heavy metal pollution on food safety in China. PLoS ONE, 10, e0131597.
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