Optimization of Plant Growth Regulators for In Vitro Shoot Multiplication of Salvadora persica L.

Authors

  • Shashikant T. Bandewar Department of Botany, Rajarshri Shahu Arts, Commerce and Science College, Pathri Tq., Phulambri, Chhatrapati Sambhajinagar, Maharashtra, India
  • Pramod S. Fuke Department of Botany, Rajarshri Shahu Arts, Commerce and Science College, Pathri Tq., Phulambri, Chhatrapati Sambhajinagar, Maharashtra, India
  • Kamble T. D. Department of Botany, Rajarshri Shahu Arts, Commerce and Science College, Pathri Tq., Phulambri, Chhatrapati Sambhajinagar, Maharashtra, India

Keywords:

Salvadora persica, Murashige and Skoog, IAA, BAP, KIN

Abstract

The present investigation was undertaken to develop an efficient protocol for the rapid micropropagation of Salvadora persica L., a medicinally and economically important desert tree. Leaf explants were cultured on Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) supplemented with different concentrations and combinations of plant growth regulators. The stem explants were excised from plant and inoculated on MS medium supplemented with 0.5 mg/L of IAA along with different concentrations of BAP and KIN. The frequency of shoot regeneration from stem node was affected by different concentrations of auxins and cytokinin. Length of shoot recorded was maximum on 0.5 mg/L IAA along with 2.5 mg/L BAP and 3.0 mg/L of KIN using stem as explant. However, at 1.5 mg/L of BAP and 2.0 mg/L of KIN concentration of showed satisfactory rate of multiplication using leaf explant.

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References

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Inventum Biologicum

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Published

03-09-2026

How to Cite

Bandewar, S. T., Fuke, P. S., & Kamble, T. D. (2026). Optimization of Plant Growth Regulators for In Vitro Shoot Multiplication of Salvadora persica L. Inventum Biologicum: An International Journal of Biological Research, 6(3), 52–55. Retrieved from https://journals.worldbiologica.com/ib/article/view/224

Issue

Vol. 6 No. 3 (2026)

Section

Research article

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