Isolation and Characterization of Halophilic Bacteria from Coastal Areas of Odisha, India

Itishree Behera; Umesh Chandra Naik

Autori

Itishree Behera Environmental Microbiology Laboratory, Department of Botany, Ravenshaw University, Cuttack-753003, Odisha, India https://orcid.org/0009-0005-3449-0516
Umesh Chandra Naik Environmental Microbiology Laboratory, Department of Botany, Ravenshaw University, Cuttack-753003, Odisha, India

Cuvinte cheie:

Isolation, Salinity, Halophilic bacteria, Biosorption, Heavy metals

Rezumat

The current study focuses on the screening and identification of halophilic bacteria from different habitats of Odisha. The halophilic bacteria were isolated and screened with NaCl concentration using 0%-24% (w/v). The selected halophiles were studied for their growth, colony morphology, multi-metal tolerance, sodium capture capacity and biochemical assay. The isolated bacteria were found to be moderately halophilic nature due to the presence of salt such as sodium, magnesium and bicarbonate in the collected samples. The result showed the utmost number of viable cells (0.022 to 1.75 cfu/mL) is due to the hypersaline nature of samples making the bacterial cells suitable to grow under 15%-18% (w/v) NaCl concentration. They were found to be gram-positive and organized in single rods and clusters. The presence of sodium (0.07-0.18 mg/L) were confirmed in the halophiles. The biochemical assay showed all strains are catalase, amylase, urease positive while showed negative results for indole test and cellulose activity. Thus, the findings suggest the existence of halophilic bacteria in the coastal areas of Odisha which can be further studied for their osmotolerant and osmoregulatory properties.

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